Fmoc-Cys(Trt)-Wang resin LL (100-200 mesh)

Fmoc-Cys(Trt)-Wang Resin LL (100–200 Mesh): Your Go-To Resin for C-Terminal Cysteine Peptide Synthesis

If you’re synthesizing peptides with a C-terminal cysteine, choosing the right resin is crucial for both yield and purity. That’s where Fmoc-Cys(Trt)-Wang Resin LL (100–200 mesh) comes in—a reliable, preloaded resin designed specifically for Fmoc solid-phase peptide synthesis (SPPS) with high loading capacity and clean cleavage properties.

This blog will walk you through everything you need to know about this resin, including its structure, benefits, applications, and tips for optimal use.

What Is Fmoc-Cys(Trt)-Wang Resin LL?

Fmoc-Cys(Trt)-Wang Resin LL is a low-loading (LL) polystyrene-based resin preloaded with Fmoc-protected cysteine, where the cysteine side chain is protected with a trityl (Trt) group. The C-terminal linkage is made through the acid-labile Wang linker, which ensures clean cleavage and yields a free carboxylic acid at the peptide’s C-terminus.

Resin Specs:

Amino Acid: Fmoc-Cys(Trt)
Linker: Wang (acid-cleavable)
Loading: Low loading (typically ~0.2–0.4 mmol/g)
Mesh Size: 100–200 mesh (ideal for swelling and flow)

Why Choose the LL (Low-Loading) Version?

Low-loading resins are especially advantageous when synthesizing:

Long or aggregation-prone peptides
Peptides with multiple cysteine residues
Sequences sensitive to steric hindrance

LL resins reduce intra- and intermolecular interactions during synthesis, which leads to:

Better coupling efficiency
Reduced deletion sequences
Improved crude purity

Key Features and Benefits

Preloaded with Fmoc-Cys(Trt): Eliminates variability and saves time
Trityl protection: Prevents unwanted side-chain reactions during synthesis
Wang linker: Produces C-terminal carboxylic acids under TFA cleavage
100–200 mesh size: Ensures optimal swelling and reagent penetration
SPPS-compatible: Ideal for automated and manual synthesis systems

Applications

this is widely used in:

Peptides with C-terminal cysteine residues
Disulfide-rich peptides or cyclic peptides
Site-specific conjugation (e.g., maleimide or thiol-reactive chemistries)
Therapeutic and diagnostic peptides
Peptidomimetics and modified biopolymers

How to Use It

1. Swelling

Pre-swell the resin in DMF or DCM for 30–60 minutes for optimal performance.

2. Synthesis

Proceed with standard Fmoc deprotection and coupling cycles using agents like HBTU/HOBt or DIC/Oxyma.

3. Cleavage

Use TFA-based cleavage cocktails (e.g., TFA/water/TIS) to remove the peptide from the resin and simultaneously deprotect the Trt group.

4. Purification

Crude peptides typically show improved purity thanks to minimized aggregation and side reactions on low-loading resin.

Storage and Stability

Storage: Keep dry at 2–8 °C in a sealed container
Shelf life: Long shelf life when stored properly
Handling: Use standard precautions for peptide synthesis reagents

Where to Buy Fmoc-Cys(Trt)-Wang Resin LL

When searching online, use SEO-friendly terms like:

Buy Fmoc-Cys(Trt)-Wang resin
Cysteine resin for peptide synthesis
Preloaded cysteine Wang resin LL
Low-loading peptide resin 100–200 mesh

Leading peptide synthesis suppliers offer this resin in bulk and research-grade quantities with a Certificate of Analysis (CoA).

Final Thoughts

Fmoc-Cys(Trt)-Wang Resin LL (100–200 mesh) is a high-performance choice for synthesizing peptides with C-terminal cysteine. Its combination of low loading, side-chain protection, and a cleavable Wang linker makes it ideal for producing high-purity peptides with minimized side reactions.

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